> ## Documentation Index
> Fetch the complete documentation index at: https://proto.evodesign.org/docs/llms.txt
> Use this file to discover all available pages before exploring further.
# miRNA Specificity
> Score predicted miRNA-mediated repression with miRanda target-site calls.
**License:** miRanda's own code is licensed under GPL-2.0, and it federates over bundled data sources and components, each under its own license terms.
This constraint is open source. Any third-party models, product names, or trademarks referenced are the property of their respective owners, and Proto is not affiliated with them.
```bibtex theme={null}
@article{enright2003microrna,
title={MicroRNA targets in Drosophila},
author={Enright, Anton J and John, Bino and Gaul, Ulrike and Tuschl, Thomas and Sander, Chris and Marks, Debora S},
journal={Genome Biology},
volume={5},
number={1},
pages={R1},
year={2003},
doi={10.1186/gb-2003-5-1-r1}
}
@article{hofacker1994fast,
title={Fast Folding and Comparison of RNA Secondary Structures},
author={Hofacker, Ivo L and Fontana, Walter and Stadler, Peter F and Bonhoeffer, L. S. and Tacker, Manfred and Schuster, Peter},
journal={Monatshefte f{\"u}r Chemie},
volume={125},
number={2},
pages={167--188},
year={1994},
doi={10.1007/BF00818163}
}
```
Configuration for miRNA target-site specificity scoring.
miRNA query sequences to scan against each target.
Optional identifiers for miRNA queries.
Optional nonnegative per-miRNA expression weights matching the miRNA query order.
Whether predicted miRNA repression should be high or low.
Options: `maximize`, `minimize`
Aggregated repression score treated as saturating success.
Alignment score used to normalize each miRanda hit.
Absolute duplex energy used to normalize each miRanda hit.
miRanda scan configuration.
evo-design/proto-language/proto\_language/constraint/sequence\_annotation/mirna\_specificity\_constraint.py
adititm