> ## Documentation Index
> Fetch the complete documentation index at: https://proto.evodesign.org/docs/llms.txt
> Use this file to discover all available pages before exploring further.

# Sequence Length

> Evaluate sequence length against target value or acceptable range

<div class="page-hero">
  <img class="page-hero-banner" src="https://proto-bio.github.io/proto-assets/images/constraint/sequence-length/hero.png" alt="Sequence Length" />
</div>

<p class="entity-disclaimer">This constraint is open source. Any third-party models, product names, or trademarks referenced are the property of their respective owners, and Proto is not affiliated with them.</p>

<hr class="entity-rule" />

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<a href="https://github.com/evo-design/proto-language/blob/d3b7822f74ea64747cc751a3b2ab1aa6b799ac47/proto_language/constraint/sequence_composition/sequence_length_constraint.py#L97" target="_blank" class="tab-panel source-panel entity-source-panel">
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    <span class="source-path">evo-design/proto-language<span class="source-subpath">/proto\_language/constraint/sequence\_composition/sequence\_length\_constraint.py</span></span>
  </div>

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<div class="entity-contributors"><span class="entity-contributors-label">Constraint contributors</span><span class="entity-contributors-people"><a class="entity-contributor" href="https://github.com/dguo8412" target="_blank" rel="noopener" title="dguo8412: 2 commits"><img noZoom class="entity-contributor-avatar" src="https://avatars.githubusercontent.com/u/46211285?v=4&s=64" alt="" loading="lazy" /><span class="entity-contributor-login">dguo8412</span></a></span></div>
Evaluate sequence length against target value or acceptable range.

This constraint function evaluates whether sequences have appropriate lengths.
It supports two modes: range mode (acceptable length window) and target mode
(exact length matching).

## API Reference

<div class="api-model-section api-model-static api-config-section">
  <div class="api-model-header"><span class="api-model-badge api-config-badge">Config</span><span class="api-model-name">SequenceLengthConfig</span><a href="https://github.com/evo-design/proto-language/blob/d3b7822f74ea64747cc751a3b2ab1aa6b799ac47/proto_language/constraint/sequence_composition/sequence_length_constraint.py#L14" target="_blank" class="func-table-btn func-source-btn api-model-source"><svg width="12" height="12" viewBox="0 0 24 24" fill="none" stroke="currentColor" stroke-width="2" stroke-linecap="round" stroke-linejoin="round"><polyline points="16 18 22 12 16 6" /><polyline points="8 6 2 12 8 18" /></svg> Source</a></div>

  Configuration for sequence length constraint.

  This class defines configuration parameters for evaluating sequence length
  in DNA, RNA, or protein sequences. The constraint supports two modes:
  range mode (specify acceptable length range) and target mode (specify exact
  target length).

  Supports two mutually exclusive modes:

  1. **Range mode**: Specify both min\_length and max\_length to define an
     acceptable length range. Sequences within this range receive score 0.0,
     while those outside are penalized based on distance from the range.
  2. **Target mode**: Specify target\_length for exact length matching. Sequences
     exactly matching the target receive score 0.0, while deviations are penalized
     based on proportional distance from the target.

  <ParamField path="min_length" type="integer">
    Minimum acceptable length (use with max\_length for range mode)
  </ParamField>

  <ParamField path="max_length" type="integer">
    Maximum acceptable length (use with min\_length for range mode)
  </ParamField>

  <ParamField path="target_length" type="integer">
    Target length for exact matching (alternative to min/max range)
  </ParamField>
</div>

<div class="api-model-section api-model-static api-output-section">
  <div class="api-model-header"><span class="api-model-badge api-output-badge">Returns</span><span class="api-model-name">ConstraintOutput</span></div>

  One result per sequence. A score of 0.0 indicates
  the sequence meets the length requirement (within range or at target).
  Higher scores indicate greater deviation:

  * **Range mode**: Linear penalty based on distance outside \[min, max].
    Score = 0.0 if within range, else proportional to deviation distance.
  * **Target mode**: Normalized penalty as |actual - target| / target.
    For example, 10% deviation from target yields score \~0.1.

  `metadata` carries:

  **For range mode:**

  * `length`: Integer actual sequence length
  * `length_mode`: String "range"
  * `length_min`: Integer minimum acceptable length
  * `length_max`: Integer maximum acceptable length

  **For target mode:**

  * `length`: Integer actual sequence length
  * `length_mode`: String "target"
  * `length_target`: Integer target length
</div>

## Usage

Range mode (protein):

```python python icon="python" theme={null}
>>> seqs = [(Sequence("MVLSP", "protein"),)]
>>> cfg = SequenceLengthConfig(min_length=4, max_length=10)
>>> results = sequence_length_constraint(seqs, cfg)
```

Target mode (DNA):

```python python icon="python" theme={null}
>>> seqs = [(Sequence("ATCGATCG", "dna"),)]
>>> cfg = SequenceLengthConfig(target_length=8)
>>> results = sequence_length_constraint(seqs, cfg)
```

## Metadata

| Property        | Value                        |
| --------------- | ---------------------------- |
| Key             | `sequence-length`            |
| Function        | `sequence_length_constraint` |
| Category        | `sequence_composition`       |
| Mode            | `discrete`                   |
| Uses GPU        | `False`                      |
| Supported Types | `dna`, `rna`, `protein`      |
