> ## Documentation Index
> Fetch the complete documentation index at: https://proto.evodesign.org/docs/llms.txt
> Use this file to discover all available pages before exploring further.

# Evo1 DNA Language Model

> Evo1 genome language model for DNA sequence generation

<div class="page-hero">
  <img class="page-hero-banner" src="https://proto-bio.github.io/proto-assets/images/generator/evo1/hero.png" alt="Evo1 DNA Language Model" />
</div>

<Note>
  **License:** Evo1 is open source and free for academic and commercial use under an Apache-2.0 license. Please refer to [the license](https://github.com/evo-design/evo/blob/main/LICENSE) for full terms.
</Note>

<p class="entity-disclaimer">This generator is open source. Any third-party models, product names, or trademarks referenced are the property of their respective owners, and Proto is not affiliated with them.</p>

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    ```bibtex theme={null}
    @article{nguyen2024evo,
      title={Sequence modeling and design from molecular to genome scale with Evo},
      author={Nguyen, Eric and Poli, Michael and Durrant, Matthew G and Kang, Brian and Katrekar, Dhruva and Li, David B and Bartie, Liam J and Thomas, Armin W and King, Samuel H and Brixi, Garyk and Sullivan, Jeremy and Ng, Madelena Y and Lewis, Ashley and Lou, Aaron and Ermon, Stefano and Baccus, Stephen A and Hernandez-Boussard, Tina and R{\'e}, Christopher and Hsu, Patrick D and Hie, Brian L},
      journal={Science},
      volume={386},
      number={6723},
      pages={eado9336},
      year={2024},
      publisher={American Association for the Advancement of Science},
      doi={10.1126/science.ado9336}
    }
    ```
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<div class="entity-contributors"><span class="entity-contributors-label">Generator contributors</span><span class="entity-contributors-people"><a class="entity-contributor" href="https://github.com/dguo8412" target="_blank" rel="noopener" title="dguo8412: 5 commits"><img noZoom class="entity-contributor-avatar" src="https://avatars.githubusercontent.com/u/46211285?v=4&s=64" alt="" loading="lazy" /><span class="entity-contributor-login">dguo8412</span></a></span></div>
Sequence generator using the Evo1 genomic language model.

Supports multiple checkpoints including CRISPR and transposon fine-tuned
variants. The number of tokens to generate is automatically calculated
based on the assigned segment's sequence\_length.

## API Reference

<div class="api-model-section api-model-static api-config-section">
  <div class="api-model-header"><span class="api-model-badge api-config-badge">Config</span><span class="api-model-name">Evo1GeneratorConfig</span><a href="https://github.com/evo-design/proto-language/blob/d3b7822f74ea64747cc751a3b2ab1aa6b799ac47/proto_language/generator/evo1_generator.py#L18" target="_blank" class="func-table-btn func-source-btn api-model-source"><svg width="12" height="12" viewBox="0 0 24 24" fill="none" stroke="currentColor" stroke-width="2" stroke-linecap="round" stroke-linejoin="round"><polyline points="16 18 22 12 16 6" /><polyline points="8 6 2 12 8 18" /></svg> Source</a></div>

  Configuration object for Evo1Generator.

  <ParamField path="prompts" type="List[string]" required>
    Prompt sequences for DNA generation (single prompt or multiple)
  </ParamField>

  <ParamField path="model_checkpoint" type="enum" default="evo-1-8k-base">
    Evo1 model variant to load (e.g. evo-1-8k-base).

    Options: `evo-1.5-8k-base`, `evo-1-8k-base`, `evo-1-131k-base`, `evo-1-8k-crispr`, `evo-1-8k-transposon`
  </ParamField>

  <ParamField path="top_k" type="integer" default="4">
    At each step, restrict sampling to the k most probable tokens.
  </ParamField>

  <ParamField path="temperature" type="number" default="1.0">
    Sharpness of the sampling distribution. Below 1 sharpens; above 1 increases diversity. Must be > 0.
  </ParamField>

  <ParamField path="prepend_prompt" type="boolean" default="False">
    Whether to prepend prompt to generation
  </ParamField>

  <ParamField path="device" type="string" default="cuda">
    GPU device to run Evo1 on (e.g. 'cuda' or 'cuda:0').
  </ParamField>

  <ParamField path="batch_size" type="integer" default="1">
    Number of sequences to process simultaneously on GPU
  </ParamField>

  <ParamField path="verbose" type="boolean" default="False">
    Whether to print verbose output
  </ParamField>
</div>

## Usage

```python python icon="python" theme={null}
>>> config = Evo1GeneratorConfig(
...     prompts="ATG",
...     model_checkpoint="evo-1-8k-crispr",
...     temperature=1.0,
... )
>>> gen = Evo1Generator(config)
>>> segment = Segment(length=1003, sequence_type="dna")
>>> gen.assign(segment)  # prepend_prompt defaults to False, so max_new_tokens = 1003
>>> gen.sample()
```

## Metadata

| Property                 | Value            |
| ------------------------ | ---------------- |
| Key                      | `evo1`           |
| Class                    | `Evo1Generator`  |
| Category                 | `autoregressive` |
| Input Type               | `prompt`         |
| Uses GPU                 | `True`           |
| Supported Sequence Types | `dna`            |
| Allows Empty Start       | `False`          |
