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σ70 promoter tuning
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sigma70 reporter expression cassette
Variable sigma70 promoter (-35 box / spacer / -10 box)
sigma70 promoter consensus score
Promoter GC content window
Promoter homopolymer cap
Promoter mutation generator
Fixed Shine-Dalgarno + 5' UTR + start codon
Fixed sfGFP coding-sequence prefix
Fixed TAA stop codon
Variable rho-independent terminator
Terminator homopolymer cap
Terminator GC-rich stem window
Terminator mutation generator
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sigma70 reporter expression cassette
Variable sigma70 promoter (-35 box / spacer / -10 box)
50 bp
Fixed Shine-Dalgarno + 5' UTR + start codon
Const
21 bp
A
A
G
G
A
G
G
A
G
A
A
A
A
T
G
C
A
T
A
…
Fixed sfGFP coding-sequence prefix
Const
84 bp
A
G
T
A
A
A
G
G
A
G
A
A
G
A
A
C
T
T
T
…
Fixed TAA stop codon
Const
3 bp
T
A
A
Variable rho-independent terminator
40 bp
sigma70 promoter consensus score
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Promoter GC content window
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Promoter homopolymer cap
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Terminator homopolymer cap
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Terminator GC-rich stem window
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Promoter mutation generator
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Terminator mutation generator
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